Part:BBa_K5038043
PT7-LIRA_input210-T7TE
Usage and Biology
LIRA_input210 is the gene sequence that was used to generate hsa-miR-210-3p. hsa-miR-210-3p can be used in response to the detection of LIRA_H210 and initiate the expression of downstream genes. The principle is the same as LIRA_input01.
This device is used to express LIRA_input210 in E.coli to test the function of LIRA_H210_2/20, LIRA_H210_7/15 and LIRA_H210_10/12
Characterization
T7 promoter could be induced by IPTG, so the intracellularly concentration of LIRA_input210 produces a large difference between the two conditions of IPTG presence/absence. This composite part is equipped by the following plasmids and co-transformed with the corresponding H210 plasmid.
(Table 1. LIRA_input210 plasmids and the corresponding H210 plasmids which have different stem/loop ratios)
No. | LIRA_input210 plasmids | corresponding H210 plasmid |
---|---|---|
1 | pCDFDuet-LIRA_input210 | pCOLADuet-H210_2/20-linker-EGFP |
2 | pCDFDuet-LIRA_input210 | pCOLADuet-H210_7/15-linker-EGFP |
3 | pCDFDuet-LIRA_input210 | pCOLADuet-H210_10/12-linker-EGFP |
4 | pET-15b-LIRA_input210 | pCOLADuet-H210_2/20-linker-EGFP |
5 | pET-15b-LIRA_input210 | pCOLADuet-H210_7/15-linker-EGFP |
6 | pET-15b-LIRA_input210 | pCOLADuet-H210_10/12-linker-EGFP |
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
None |